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Key documents for increased AMR control

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Background of AMR
Rapid tests / PoCT

Publications on evaluation of LFIA devices from NG-Biotech


Fenwick AJ, Bergman Y, Lewis S, Yee R, Uhlemann AC, Cole N, Kohner P, Ordak C, Green DA, Schuetz AN, Humphries R, Simner PJ. Evaluation of the NG-Test MCR-1 Lateral Flow Assay and EDTA-Colistin Broth Disk Elution Methods To Detect Plasmid-Mediated Colistin Resistance among Gram-Negative Bacterial Isolates. J Clin Microbiol. 2020 Mar 25;58(4):e01823-19. doi: 10.1128/JCM.01823-19. Print 2020 Mar 25.

Volland H, Dortet L, Bernabeu S, Boutal H, Haenni M, Madec JY, Robin F, Beyrouthy R, Naas T, Simon S. Development and Multicentric Validation of a Lateral Flow Immunoassay for Rapid Detection of MCR-1-Producing Enterobacteriaceae. J Clin Microbiol. 2019 Apr 26;57(5):e01454-18. doi: 10.1128/JCM.01454-18.


Moguet C, Gonzalez C, Naas T, Simon S, Volland H. Multiplex Lateral Flow Immunoassay for the Detection of Expanded-Spectrum Hydrolysis and CTX-M Enzymes. Diagnostics. 2022; 12(1):190.

Gonzalez C, Moguet C, Chalin A, Oueslati S, Dortet L, Simon S, Volland H, Naas T. Comparison of Three Expanded-Spectrum Cephalosporin Hydrolysis Assays and the NG-Test CTX-M Multi Assay That Detects All CTX-M-Like Enzymes. Diagnostics. 2022; 12(1):197.

Bernabeu S, Ratnam KC, Boutal H, Gonzalez C, Vogel A, Devilliers K, Plaisance M, Oueslati S, Malhotra-Kumar S, Dortet L, Fortineau N, Simon S, Volland H, Naas T. A Lateral Flow Immunoassay for the Rapid Identification of CTX-M-Producing Enterobacterales from Culture Plates and Positive Blood Cultures. Diagnostics (Basel). 2020 Sep 28;10(10):764. doi: 10.3390/diagnostics10100764

Bianco G, Boattini M, Iannaccone M, Cavallo R, Costa C. Evaluation of the NG-Test CTX-M MULTI immunochromatographic assay for the rapid detection of CTX-M extended-spectrum-beta-lactamase producers from positive blood cultures. J Hosp Infect. 2020 Jun;105(2):341-343. doi: 10.1016/j.jhin.2020.02.009. Epub 2020 Feb 21.

VanA – VanB

Saoussen Oueslati, Herve VOLLAND, Vincent Cattoir, Sandrine Bernabeu, Delphine Girlich, duncan dulac, Marc Plaisance, laure boutigny, Laurent Dortet, Stephanie Simon, and Thierry Naas. Rapid Detection of VanA/B-Producing Vancomycin-Resistant enterococci using Lateral Flow Immunoassay from colonies and blood culture (pre-print server: BIORXIV/2020/395293). Submitted to mSphere

Oueslati S, Volland H, Cattoir V, Bernabeu S, Girlich D, Dulac D, Plaisance M, Laroche M, Dortet L, Simon S, Naas T. Development and Validation of a Lateral Flow Immunoassay for Rapid Detection of VanA-Producing Enterococci. J Antimicrob Chemother. 2020 (in press)


Baeza LL, Pfennigwerth N, Greissl C, Göttig S, Saleh A, Stelzer Y, Gatermann SG, Hamprecht A. Comparison of five methods for detection of carbapenemases in Enterobacterales with proposal of a new algorithm. Clin Microbiol Infect. 2019 Oct;25(10):1286.e9-1286.e15. doi: 10.1016/j.cmi.2019.03.003.

Khalifa HO, Okanda T, Abd El-Hafeez AA, El Latif AA, Habib AGK, Yano H, Kato Y, Matsumoto T. Comparative Evaluation of Five Assays for Detection of Carbapenemases with a Proposed Scheme for Their Precise Application. J Mol Diagn. 2020 Sep;22(9):1129-1138. doi: 10.1016/j.jmoldx.2020.05.012.

Kanahashi T, Matsumura Y, Yamamoto M, Tanaka M, Nagao M. Comparison of the Xpert Carba-R and NG-Test CARBA5 for the detection of carbapenemases in an IMP-type carbapenemase endemic region in Japan. J Infect Chemother. 2020 Nov 17:S1341-321X(20)30408-6. doi: 10.1016/j.jiac.2020.11.001.

Ben-Haim O, Azrad M, Saleh N, Tkhawkho L, Peretz A. Evaluation of the NG-Test CARBA 5 Kit for Rapid Detection of Carbapenemase Resistant Enterobacteriaceae. Lab Med. 2020 Nov 7:lmaa084. doi: 10.1093/labmed/lmaa084.

Gelmez GA, Can B, Hasdemir U, Soyletir G. Evaluation of two commercial methods for rapid detection of the carbapenemase-producing Klebsiella pneumoniae. J Microbiol Methods. 2020 Oct 10;178:106084. doi: 10.1016/j.mimet.2020.106084.

Stokes W, Pitout J, Campbell L, Church D, Gregson D. Rapid detection of carbapenemase-producing organisms directly from blood cultures positive for Gram-negative bacilli. Eur J Clin Microbiol Infect Dis. 2020 Aug 11. doi: 10.1007/s10096-020-04005-4.

Chan WW, Campbell L, Doyle D, Pitout JD. Rapid detection of Enterobacterales that produce carbapenemases. Diagn Microbiol Infect Dis. 2020 Oct;98(2):115120. doi: 10.1016/j.diagmicrobio.2020.115120. Epub 2020 Jun 26.

Jenkins S, Ledeboer NA, Westblade LF, Burnham CA, Faron ML, Bergman Y, Yee R, Mesich B, Gerstbrein D, Wallace MA, Robertson A, Fauntleroy KA, Klavins AS, Malherbe R, Hsiung A, Simner PJ. Evaluation of NG-Test Carba 5 for Rapid Phenotypic Detection and Differentiation of Five Common Carbapenemase Families: Results of a Multicenter Clinical Evaluation. J Clin Microbiol. 2020 Jun 24;58(7):e00344-20. doi: 10.1128/JCM.00344-20.

Bianco G, Boattini M, van Asten SAV, Iannaccone M, Zanotto E, Zaccaria T, Bernards AT, Cavallo R, Costa C. RESIST-5 O.O.K.N.V. and NG-Test Carba 5 assays for the rapid detection of carbapenemase-producing Enterobacterales from positive blood cultures: a comparative study. J Hosp Infect. 2020 Jun;105(2):162-166. doi: 10.1016/j.jhin.2020.03.022.

Bogaerts P, Berger AS, Evrard S, Huang TD. Comparison of two multiplex immunochromatographic assays for the rapid detection of major carbapenemases in Enterobacterales. J Antimicrob Chemother. 2020 Jun 1;75(6):1491-1494. doi: 10.1093/jac/dkaa043.

Ratnayake L, Ang HZ, Ong CH, Chan DSG. An optimized algorithm with improved turnaround time for detection of carbapenemase-producing Enterobacterales using the NG Test CARBA 5 in a routine laboratory. J Med Microbiol. 2020 Feb;69(2):228-232. doi: 10.1099/jmm.0.001132.

Volland H, Girlich D, Laguide M, Gonzalez C, Paris V, Laroche M, Oueslati S, Dortet L, Simon S, Naas T. Improvement of the Immunochromatographic NG-Test Carba 5 Assay for the Detection of IMP Variants Previously Undetected. Antimicrob Agents Chemother. 2019 Dec 20;64(1):e01940-19. doi: 10.1128/AAC.01940-19.

Potron A, Fournier D, Emeraud C, Triponney P, Plésiat P, Naas T, Dortet L. Evaluation of the Immunochromatographic NG-Test Carba 5 for Rapid Identification of Carbapenemase in Nonfermenters. Antimicrob Agents Chemother. 2019 Aug 23;63(9):e00968-19. doi: 10.1128/AAC.00968-19.

Giordano L, Fiori B, D’Inzeo T, Parisi G, Liotti FM, Menchinelli G, De Angelis G, De Maio F, Luzzaro F, Sanguinetti M, Posteraro B, Spanu T. Simplified Testing Method for Direct Detection of Carbapenemase-Producing Organisms from Positive Blood Cultures Using the NG-Test Carba 5 Assay. Antimicrob Agents Chemother. 2019 Jun 24;63(7):e00550-19. doi: 10.1128/AAC.00550-19.

Takissian J, Bonnin RA, Naas T, Dortet L. NG-Test Carba 5 for Rapid Detection of Carbapenemase-Producing Enterobacterales from Positive Blood Cultures. Antimicrob Agents Chemother. 2019 Apr 25;63(5):e00011-19. doi: 10.1128/AAC.00011-19.

Hopkins KL, Meunier D, Naas T, Volland H, Woodford N. Evaluation of the NG-Test CARBA 5 multiplex immunochromatographic assay for the detection of KPC, OXA-48-like, NDM, VIM and IMP carbapenemases. J Antimicrob Chemother. 2018 Dec 1;73(12):3523-3526. doi: 10.1093/jac/dky342.

Publications on using of LFIA devices from NG-Biotech

Delphine Girlich, Rémy A. Bonnin, and Thierry Naas. Occurrence and diversity of CTX-M-producing Escherichia coli from the Seine river. Frontiers in Microbiology (in press)

Dabos L, Bogaerts P, Bonnin RA, Zavala A, Sacré P, Iorga BI, Huang DT, Glupczynski Y, Naas T. Genetic and Biochemical characterization of OXA-519, a novel OXA-48-like β-lactamase. Antimicrob Agents Chemother. 2018 Jun 4. pii: AAC.00469-18. doi: 10.1128/AAC.00469-18. [IF= 4.9]


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